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Family business is a predominant form of business in many countries of the world, it becomes discussed and current topic in the Czech Republic. Small and medium family businesses have long been the backbone of the world economy, in a globalized economy they are in competition with large multinationals, above all by their flexibility. Understanding and knowledge the specifics of family businesses, applying a parallel process of their strategic management, including human resources management, an optimally defined organizational structure, ability of managing conflicts can impact on their competitiveness and success in a globalized economy. The subject of scientific interest is family business as an economic subject. The purpose of this paper is to identify the specifics of human resource management in family business together with respecting and intermingling dimensions of family, ownership and business. This paper is elaborated by the method of collecting and analyzing information, literature search, compilation and critical evaluation of expert texts. In addition, primary quantitative research, qualitative research was used, information from group discussions with members of family businesses was evaluated. It can be assumed that the specifics of human resources management are based on a virtually inseparable boundary between family business and the family, their interdependent and intertwining relationships, the ability to manage conflicts. It has been shown that these surveyed family businesses are using subsidies provided by the Czech Labor Offices to educate family members and employees, it is very important to meet and exchange experiences between founders and successors of family businesses and to explore them through a university platform.